Nutraceutical and Dietary Supplement Testing

Nutraceutical and dietary supplements are already nearly a $100 billion industry worldwide. The fact that nutrition and health go hand-in-hand and is not a new concept. More than 2,000 years ago Hippocrates is credited with stating “If we could give every individual the right amount of nourishment and exercise, not too little and not too much, we would have found the safest way to health.” We also provide product testing of novel compounds, routine raw material quality assurance testing, and label guarantee using AOAC, USP, FCC and other methods.  A few of our services are listed below. If you don’t see what you need, contact us.

aloeAloe

Aloe vera has received a stamp of approval in human health for the ability of the plant and plant gel to treat burns and has been widely used in topical sunburn creams. Studies in the Netherlands have examined the immune activity of Aloe. Not surprisingly, a highly active polysaccharide fraction of the Aloe gel was isolated and local activation of the complement cascade led to an influx of monocytes and poly morphonucleotides (PMN) to injuries was noted (Davis et al, 1989). A trial published in November of 2005 found that aloe vera improved the absorption of both vitamins C and E (Vintson et al., 2005). Aloe vera consists of a glucomannan polysaccharide.

Services Provided:
Aloe testing by USP Methods:

  • Water content of powder products.
  • Total Ash
  • Alcohol Insoluble substances
  • Percent glucose and mannose (by HPLC)
  • Quantification of Aloe polysaccharides by JAOAC method (88:685-691. 2005).

Alpha Lipoic Acid

α-Lipoic acid (also known as thioctic acid) has been documented as an enzyme cofactor and an antioxidant.

Services Provided:
Identification and Quantification of α-Lipoic acid by HPLC (USP-NF Method).

Anthocyanins

These flavonoid pigments are found in plants, providing color in everything from fruits to autumn leaves. More than 550 different anthocyanins have been identified. Anthocyanins act as powerful antioxidants that aid in protecting the plant from free radicals formed during metabolic processes.

Services Provided:
Total monomeric Anthocyanin by pH differential method.
HPLC characterization and quantification.
Contact us for the availability of analysis of specific anthocyanins

Beta Glucan Content (Cereal grains): (AOAC Method)

Mixed linkage β-glucans are polymers of 1-3, 1-4 β-glucose molecules and is a component of barley and oats.  Β-glucans have been shown to regulate blood glucose levels, lower serum cholesterol and modulate the immune system.  The FDA approved a health claim for oat products containing 0.75-grams of β-glucan per serving indicating these levels can reduce the risk of heart disease (FDA, 1997).

Services Provided:
(AOAC Method for cereal Beta Glucans)

Beta Glucan Content (Yeast, Fungi and Algae): (1-3)-β-glucans are widely distributed in nature, especially in algae, fungi and yeast, and also in higher plants.  Venture Labs offers 1,3:1,6-beta-glucan specific testing as well as quantifying α-glucan in preparations.   Contact Us for assays to address your specific needs.   

 

cranberriesCranberry

The USDA lists cranberries as number 6 on a list of 20 common foods with high antioxidant content. Antioxidants protect the body from free radical damage. Reductions in LDL cholesterol, possible cancer protective effects and improvements in urinary tract health have also been attributed to cranberries (Ruel et al., 2005; Ferguson et al., 2004; Sun et al., 2004; Howell et al., 2005).

Services Provided:
Dextrose and Fructose content (USP Method)
Organic Acid Content (USP Method)
Refractive Index (USP Method)
Sorbitol and Sucrose content (USP Method)

 

Enzyme Supplements and Preparations

Fatty Acids in Fish Oils

A number of studies have shown an inverse relationship between fish oil consumption and various forms of cancer and risk of cardiovascular disease.  Marine life is generally a rich source of the omega-3 fatty acids EPA and DHA.

Services Provided:
Eicosapentaenoic acid and Docosahexaenoic acid (EPA and DHA) (Gas Chromatography – AOAC Method)

Glucomannan from Konjac Flour

Konjac flour is a hydrocolloidal polysaccharide from the tubers of Amorphophallus species of plants. It is a glucomannan polysaccharide consisting of β-1,4 linked glucose and mannose with a molar ratio of 1.6(mannose):1.0(glucose). It is commonly used as a diet aid, gelling agent, thickener, and emulsifier.

Services Provided:
Food Chemicals Codex microscopic test
FCC Gel Test
FCC Heat Stable gel test
FCC Total carbohydrate (by subtraction)
Percent glucose and mannose (by HPLC)

Flavonols

An important source of antioxidant compounds the flavonol glycosides are water-soluble pigments responsible for the yellowish hues of cereal grains, fruits and vegetables. Among the most prominent flavonol glycosides are Quercetin, Resveratrol, Gallic acid and Ellagic acid.

Services Provided:
HPLC analysis of many Flavonol glycosides using USP-NF methods. Contact us for the availability of analysis of specific flavonol compounds.

Glucosamine

One of the most popular dietary supplements, glucosamines have been proven to have beneficial effects on osteoarthritis (Reginster et al., 2001). Glucosamine is an amino-monosaccharide found in and around cartilage cells. Glucosamine hydrochloride and sulfate are the forms most commonly found in dietary supplements and claimed as ingredients on the label.

Services Provided:
Glucosamine content (AOAC Method)

Inulin

Inulin and oligofructose occur in foods such as chicory, garlic, onion, artichoke, asparagus, and leeks.  The associated fructans are fermented by desirable bacteria such as Bifidobacteria.  It is thought that a number of health benefits are associated with increased levels of Bifidobacteria including reductions in Clostridia and coliform bacteria and antimutagenic and anticarcinogenic properties (Sparkes et al., 1998; Reddy, 1999).

Services Provided:
USP Method of Analysis

Organic Acids

Acetic, Citric, Formic, Oxalic, Succinic and Tarataric acid.

Services Provided:
Identification and quantification by HPLC.

Polyphenolic Compoundscranberries

The polyphenolics are important secondary metabolites produced by plants. Pholyphenolics are responsible for the color and flavor of plant materials. Many have been found to have strong antioxidant and anticancer activities.

Services Provided:
Total polyphenolic compounds – By Folin-Ciocaltaeu Colorimetry. Contact us for the availability of analysis of specific Polyphenolic compounds.

 

Probiotics

With nearly 30 years experience studying probiotics, Venture Labs can handle any product assays or issues. 

Total Dietary Starch

Services provided:
(AOAC Method)

Total Starchgrapes

Services provided:
(AOAC Method)

Sugar Alcohols

Mannitol, Xylitol, erythritol, L-arabinitol, galactitol, and sorbitol.

Services Provided:
Identification and quantification by Food Chemicals Codex methods.

Vitamin Analysis

Water Soluble: Ascorbic acid (Vitamin C), Cyanocobalamine (B12), Folic acid, Niacinamide, Pantothenic acid, Para-aminobenzoic acid, thiamine.

Fat Soluble: Vitamin A, A acetate, Vitamin D2, D3, Vitamin E, E acetate and Vitamin K.

Services Provided:
Quantification by American Association of Cereal Chemists methods, HPLC and AOAC methods.

Volatile Fatty Acids

(Formic, Acetic, Propionic, Butyric, Isobutyric, Valeric, Isovaleric acids)

Services Provided:
Quantification by HPLC or Gas Chromatography.

 

References:

Davis, R.H. et al., 1989. Wound healing: Oral and topical activity of Aloe vera. J. Amer. Podiatric Medical Assoc. 79:559-562.
Ferguson, P.J., E. Kurowska, D.J. Freemena, A.F. Chambers, and D.J. Koropatnick. 2004. A flavanoid fraction from cranberry extract inhibits proliferation of human tumor cell lines. J. Nutr. 134:1529-1535.
Howell, A.B., J.D. Reed, C.G. Kreuger, R. Winterbottom, D.G. Cunningham, and M. Leahy. 2005. A-type cranberry proanthocyanidins and uropathogenic bacterial anti-0adhesion activity. Phytochemistry. 66:2281-2291.

Reddy, B.S.  1999.  Possible mechanisms by which Pro- and prebiotics influence colon carcinogenesis and tumor growth.  J. Nutr. 1478S-1482S.
Reginster, J., R. Deroisy, L. Rovati, R. Lee, E. Lejeune, O. Bruyere, G. Giacovelli, Y. Henrotin, J. Dacre, C. Gossett. 2001. Long term effects of Glucosamine sulfate on osteoarthritis progression: A randomized, placebo-controlled clinical trial. The Lancet. 357:251-256.
Ruel, G., S. Pomerleau, P. Coulture, B. Lamarche and C. Couillard. 2005. Changes in plasma antioxidant capacity and oxidized low-density lipoprotein levels in men after short-term cranberry juice consumption. Metabolism. 54:856-861.
Sparkes, A.H., K. Papasouliotis, G. Sunvold, G. Werrett, E.A. Gruffydd-Jones, K. Egan, T.J. Gruffydd-Jones, G. Reinhart.  Effect of dietary supplementation with fructo-oligosacchairdes on fecal flora of healthy cats.  Am. J. Vet. Res.  59:436-440.

Sun, J., Y.F. Chu, W. We, and R.H. Liu. 2002. Antioxidant and antiproliferative activities of common fruits. J. Agric. Food Chem. 50:7449-7454.
Vinson, J.A., H. Al Kharrat, L. Andreoli. 2005. Effect of Aloe vera preparations on the human bioavailability of vitamins C and E. Phytomedicine. 12:760-765.