Enzyme Assays

Venture Laboratories offers quantitative analysis of enzyme levels in a variety of matrices ranging from industrial fermentation concentrated preparations, to low levels in food ingredients such as honey. Low levels of enzymes in spices, flour and food ingredients can affect thickening agents in soups and salad dressings. We offer the following routine assays from the Food Chemicals Codex (FCC), United States Pharmacopoeia (USP) or AOAC:

Venture Labs Assay Services Provided:
  • Alpha Amylase (Non-bacterial) Activity – Dextrining units (DU).  FCC Method.
  • Alpha Amylase (Bacterial) Activity – Bacterial amylase units (BAU).  FCC Method.
  • Alpha Galactosidase Activity – Galactosidase activity units (GalU).  FCC Method.
  • Beta-Glucanase Activity – Beta Glucanase Unit (BGU).  FCC Method.
  • Bromelain Activity – (GDU) – Supplier Specific Method.
  • Catalase Activity – Baker Units (BU).  FCC Method.
  • Cellulase Activity – Cellulase Units (CU).  FCC Method.
  • Ceralpha Amylase – Ceralpha Units.  AACC #22-02.01 Method.
  • Chymotrypsin Activity – USP Chymotrypsin Unit.  FCC and USP Method.
  • Diastatic Activity of Honey – Diastase Number (DN).  AOAC Method 958.09.
  • Glucoamylase Activity – (GA unit).  FCC Method.
  • Glucose Oxidase Activity – Glucose Oxidative Titrimetric unit (GOTu).  FCC Method.
  • Hemicellulase Activity – Hemicellulase Units (HCU).  FCC Method.
  • Invertase Activity (SU) – FCC Method.
  • Lactase (Acid Beta Galactosidase) Activity – Acid Lactase Units (ALU).  FCC Method.
  • Lactase (Neutral Beta Galactosidase) Activity – Neutral Lactase Units (NLU).  FCC Method.
  • Lipase Activity – FIP Units (FIP unit).  FCC Method.
  • Lipase Activity – (LU).  FCC Method.
  • Lysozyme Activity – Various Methods.
  • Malt Diastase (Diastatic Power) – FCC Method.
  • Nattokinase Assay – Fibrinolytic Units (FU). Japan Bioscience Laboratories Method.
  • Pancreatin (Amylase, Protease and Lipase) Activity – USP Method.
  • Pancreatin Amylase – Starch Digestive Power.  USP Units.   USP Method.
  • Pancreatin Lipase – Fat Digestive Power.  USP Units.  USP Method
  • Pancreatin Protease – Casein Digestive Power. USP units.  USP Method.
  • Papain Activity – USP Method.
  • Pectinase Activity – Methods in Enzymology and Supplier Specific Methods.
  • Pectin methyl Esterase – Pectinesterase – Methods in Enzymology.
  • Pepsin Activity – FCC Method.
  • Pepsin Activity (HUT) – USP Pending Method.
  • Peroxidase Assay –  Methods in Enzymology and AACC Methods.
  • Phytase Activity (FTU) – FCC Method.
  • Polyphenol Oxidase Activity. (PPO) AACC Method
  • Polyphenol Oxidase Activity (PPO)  J. Food Sci. 71:51-58.
  • Protease (Bacterial) Activity – Bacterial Protease Unit (PC).  FCC Method.
  • Protease (Fungal) Activity – Hemoglobin Units on a Tyrosine Basis (HUT).  FCC Method.
  • Protease (Plant) Activity – Papain Unit (PU).  FCC Method.
  • Serrapeptase Activity – Japan Pharmacopeia Method.
  • Superoxide Dismutase (MFU) – McCord, J. M. and Fridovich, I. (1969) J. Biol. Chem. 244, 6049 6055.
  • Trypsin Activity – USP Trypsin Units.  FCC and USP.
  • Trypsin Inhibitor Assay – AACC Method.
  • Xylanase Activity – Bailey and Pouanen, 1989.
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We offer many other assay options or will assist you with method development appropriate to your application.

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